Eukaryotic messenger RNA undergoes several processing steps after transcription

Question

Eukaryotic messenger RNA undergoes several processing steps after transcription before it binds to ribosomes as the template for protein synthesis. One of these steps is polyadenylation. Identify whethe the statements below about polyadenylation are true or false. 1. A polymerase adds a polyadenylate tail of up to 250 nucleotides to the 5true end of the mRNA true false 2. A polymerase adds a polyadenylate tail to the mRNA while it is still in the nucleus false 3. The polymerase forming the polyadenylate tail uses a polydT DNA template strand. true false 4. The polyadenylate tail helps to protect the mRNA from exonucleases 5. Polyadenylation can occur before or after the mRNA is spliced The mKNA tor wha: eukaryoio proteing are not potyadenylated?

Explanation / Answer

1. False

Poly A polymerase is the enzyme that adds A residues to 3' end of the mRNA

2.True

The poly A tail is added to the mRNA before its transport from nucleus to cytoplasm.

3. False

The poly A tail is added in a template independent manner.

4. True

The poly A protects against degradation of mRNA from exonucleases.

5. Polyadenylation occurs before splicing of the mRNA.

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