5.) TRUE/FALSE (1 point for each correct answer) DNA Replication: Several protei

Question

5.) TRUE/FALSE (1 point for each correct answer) DNA Replication: Several proteins collaborate with DNA polymerase to replicate DNA: a.) Helicase separates double stranded DNA into two single strands b.) Clamp Loader loads the DNA polymerase onto the DNA c.) Primase generates a RNA primer required by the DNA polymerase to initiate DNA polymerization d.) Ligase connects Tonegawa fragments on the Leading strand ein Translation: Several proteins collaborate with the ribosome to translate mRNA into protein a.) EF-Tu enforces proper Codon/Anticodon pairing b.) EF-G catalyzes the peptidyl-transfer reaction c.) 64 distinct Aminoacyl-tRNA synthetases charge tRNAs with the Aminoacids specific to their anticodons d.) Several steps in Translation require a GTP/GDP switch

Explanation / Answer

Q.5

DNA Replication:

a.) True. [Explanation: Helicase unwinds dsDNA into ssDNA]

b.) True. [Explanation: Clamp loader prevents the dissociation of DNA polymerase from the template DNA]

c.) True. [Explanation: RNA primer is synthesized by Primase during DNA replication]

d.) False. [Explanation: Ligase ligate the okazaki fragments of DNA]

Protein Translation:

a.) True. [Explanation: EF-Tu catalyse the binding of aa-tRNA to ribosome, thus enforcing proper codon/anticodon pairing]

b.) True. [Explanation: EF-G is an elongation factor]

c.) False. [Explanation: There are only 20 different aa-tRNA synthetase]

d.) True. [Explanation: Most of the step including EF-Tu binding to the aa-tRNA requires GTP/GDP switch]

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