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solution 4.26 Aerobic sugar metabolism Candida stellata is a yeast frequently fo

ID: 276606 • Letter: S

Question

solution

4.26 Aerobic sugar metabolism Candida stellata is a yeast frequently found in wine fermentations. Its sugar metabolism is being studied under aerobic conditions. In continuous culture with fructose (CH1206) as the carbon source and ammonium phosphate as the nitrogen source, the yield of biomass from fructose is 0.025 gg1, the yield of ethanol (C2H&O;) from fructose is 0.21 g g1, and the yield of glycerol (C3H8O3) from fructose is 0.07gg. (a) If the rate of sugar consumption is 190 g h 1, what is the rate of oxygen consumption in g h ? (b) What is the RQ for this culture?

Explanation / Answer

Answer. The fructose is used as a building material for the formation of biomass,ethanol and glycerol. It is also used as energy source. From the given situation we find that out of 1 gm of fructose, 0.3 gm are used in biosynthesis while 0.7 gm is used in energy production. Fructose is used just like glucose. For complete oxidation of 1 mole of fructose, 6 moles of oxygen are needed. Since fructose is pumped at 190 g/hr, so 1.05 moles are consumed by Candida stella.In other words, 202.56gm of oxygen are needed for energy production from 1.05 moles of fructose. Since only 0.7 gm are used for energy production, so 141.92 gm of oxygen is needed. Answer the respiratory quotient is the ratio of volume of carbon dioxide released and oxygen consumed. RQ= Vol of CO 2/ Vol of O2 = 6/6 = 1