After observing the results from the blood agar and MSA plates, determine the ba

Question

After observing the results from the blood agar and MSA plates, determine the bacterium which formed each colony. If you cannot determine the bacterium, state that it is still unknown.

Bacterium B Gram positive a-hemolytic Bacterium C Gram positive y-he molytic Bacterium D Bacterium E Gram negtive Gram negative Gram negative -hemolytic Bacterium F Bacterium A Gram positive a-hemolytic ? hemolytic y-hemolytic facultative anaerobe obligate anaerobe obligate aerobe obligate aerobe obligate aerobe facultative anaerobe ferments glucose, ferments glucose, ferments glucose ferments glucose ferments glucose ferments glucose sucrose and lactose sucrose and lactose only acid) only (acid) and sucrose and sucrose acid & gas)(acid & gas) ferments mannitol ferments mannitol does not fermentdoes not ferment acid) (acid& gas) does not ferment does not ferment (acid) (acid) mannitol mannitol mannitol mannitol

Explanation / Answer

Colony 1, 4, 5, 7,10, 11, 13, 16 and 17 are Staphylococcus aures

Because they give beta hemolysis zone on the blood agar plate and fermented the mannitol in MSA plate which is specific character of S.au.

Colony 3, 9 and 15 are Staphylococci epidermis as they are non mannitol fermenting cultures showing gamma hemolysis.

Rest of the colonies are non hemolytic, non mannitol fermenting are non pathogenic Staphylococci species.

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