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Do Not Leave Marks o DIFFERENTIAL STAINING CAN BE USED FOR BACTERIAL IDENTIFICAT

ID: 277001 • Letter: D

Question

Do Not Leave Marks o DIFFERENTIAL STAINING CAN BE USED FOR BACTERIAL IDENTIFICATION BECAUSE OF TEMPORARY OR PERMANENT CHANGES OBSERVED AT EACH STAGE OF THE PROCESS 15) A decolorizing agent will cause both positive and negative bacteria to A) lose color B) look alike C) be different D) keep the first dye Type: MC Page Ref: 67-73 Topic: differential staining Skill: Requires Analysis 16) After the Mordant is added and rinsed off with water, positive and negative cells will A) appear different B) be colorless C) keep the color of the mordant D) look alike Type: MC Page Ref: 67-7 Topic: differential staining Skill: Requires Analysis 17) Identify the bacterium that was decolorized during Gram's experiment with dyes A) Staphylososcus aureus B) Klebsiella pneumonia C) Escherichia sall Type: MC Page Ref: 67-71 Topic: bacterial types Skill: Requires Recollection 18) A non-toxigenic microbe A) does not rely on toxins for its virulence B) is normal flora and non-pathogenic C) can produce poisonous substances D) is non-virulent and non life-threatening Type: MC Page Ref: 436-442 Topic: Skill: Requires Analysis 19) Bacteria can acquire the genes for exotoxin production through Phages, which are A) Extra additional pieces of DNA in their cells 8) The C) Immune cells that consume bacteria and other germs Dj Viruses that main circular Bacterial Chromosomes with thousands of genes t infect and destroy bacterial cells Type: MC Page Ref: 79-94 Topic: Toxins Skill: Demonstrate Understanding

Explanation / Answer

Hi Answer:

Q.15. A decolorizing agent will cause both positive and negative bacteria to

Answer: Option A (Lose color)

Explanation: discoloration is carried out by the alcohol which washed away the dye so during this step the both gram +ve or –ve organism lose color.

Q. 16.After the mordent is added and rinsed off with water, positive and negative cells will

Answer: Option A (Appear different)

Explanation: When we add mordent i.e. iodine it form complex with the crystal violet dye and after that decolourization is carried out which brings the difference in bacteria

Q. 17. Identify the bacterium that was decolorized during gram experiment with dyes

Answer: Option B& C (Klebsiella pneumonia and Escherichia coli)

Explanation: These both are garm negative in nature and was decolorized in staining experiments.

Q.18. A non-toxigenic microbe

Answer: Option D. (Is non-virulent and non-life threatening)

Explanation: The non-toxigenic microbe is neutral microorganism and it didn’t cause any virulence.

Q.19. Bacteria can acquire the genes for exotoxin production through Phages, which are

Answer: Option D (Viruses that infect and destroy bacterial cells)

Explanation: bacteriophages are the viruses which infect the bacteria.

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