3. (4 points total) Below is a copy of the coding strand of DNA for a gene. CTGA

Question

3. (4 points total) Below is a copy of the coding strand of DNA for a gene. CTGATGCAGCCGCATCGCAGATGCGTAGTTCATTTGCTGGATAACGACAGCGCTAT 3 a. (0.5 points) Draw out the non-coding strand of DNA underneath the coding strand (please show the DNA molecule sequence-wise as it would actually be) b. (0.5 points) Write out the non-coding strand of DNA below using proper conventions. c. (1 point) Please state where the promoter and transcriptional unit are located in the gene above d. (1 point) Please transcribe the gene into mRNA e. (1 point) Please take the mRNA from question d and translate it using the provided codon table

Explanation / Answer

Please find the answers below:

Answer a: The coding and non-coding strands of the DNA are the non-template and template strands, respectively. The appropriate strands can be found as below:

5' CTGATGCAGCCCGCATCGCAGATGCGTAGTTCATTTGCTGGATAACGACAGCGCTAT 3' CODING STRAND

3' GACTACGTCGGGCGTAGCGTCTACGCATCAAGTAAACGACCTATTGCTGTCGCGATA 5' NON-CODING STRAND

Answer b: The non-coding strand of the parent DNA with proper concentions on its orientations can be found as below:

3' CACTACGTCGGGCGTAGCGTCTACGCATCAAGTAAACGACCTATTGCTGTCGCGATA 5'

Answer c: The location of promoter in the non-coding strand can be found as below:

3' GACTACGTCGGGCGTAGCGTCTACGCATCAAGTAAACGACCTATTGCTGTCGCGATA 5'

Replication of DNA takes place from 5' to 3' direction, hence from left to right on the above template strand. The bold region represents the promoter region in this DNA.

Answer d: The mRNA transcript formed from this DNA would be found as below:

3' GACUACGUCGGGCGUAGCGUCUACGCAUCAAGZAAACGACCUAUUGCUGUCGCGAUA 5'

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