6) One of your colleagues is brainstorming on how to use the Flp-FRT system to s

Question

6) One of your colleagues is brainstorming on how to use the Flp-FRT system to selectively remove gene X from serotonergic cells in adult flies. She wants to know how the absence of this gene will affect serotonergic neurotransmission in the fly brain. She has drawn out two models on how to build the transgenic flies and would like your opinion (see Figure 1). Here you see the use of a two component system to delete certain exons from gene X (shown in grey). After a quick glance you realize that one of these models will probably not work to remove protein X (as a product of gene X) in adult fly brains. In your estimation which transgenic fly is flawed? What are two things that are wrong with the system as designed in that transgenic fly? Along those lines, how is the other transgenic fly better designed? Last, how could you further improve that transgenic fly? What is your rationale behind the improvement?

Explanation / Answer

Ans) In my opinion and understanding all of, system B is likely to work and is more flawed due to various reasons. With this system since the promoter is missing, it is less likely to activate the gene that is required for serotonergic cells expression or the gene expression. It is because this does not contain the promoter that is required for starting the expression.

The two things that are wrong in the system are:-

The other transgenic system or fly is designed better as it has a serotenergic cell promoter. This helps in activation of the genes without any added requirement. The other transgenic fly is better designed as it has the serotenergic cell promoter which is required for the promotion of the gene.

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