TECHNIQUES Interpret results for the following instruments used in the Microbiol

Question

TECHNIQUES Interpret results for the following instruments used in the Microbiology Lab Microscopy: Name Microscope parts and their functions Demonstrate how total magnification is calculated Recall the function of Hemocytometer in Cell count Culture media: Identify plates of Nutrient Agar, Casein Agar, Starch Agar D Eosin Methylene Blue, and agar slants O Cultures: techniques for streak plates O How to use Spectrophotometer and Microbial Growth making and keeping cultures CONTROLLING MICROBIAL GROWTH Interpret how the following affect Microorganisms 0 Heat/Temperature Disinfectants O Antibiotic sensitivity O Radiation: Damage by and protection from Ultraviolet light STAINS & STAINING Recall Stains and Reagents used for Gram Stain 0 Acid-fast stain Negative staining and the Capsule Stain Endospores stairn ? You may also be required to observe slides and interpret Positive or negative results and identify the colors

Explanation / Answer

1. Microscope parts :-    Eye piece - the viewer looks through to view the specimen Diopter adjustment:- to correct any visual difference between two eyes Body tube :- it connects the eye piece to the objective lens Arm:-it connects the body to base of the microscope Coarse adjustment:- brings specimen to general focus Fine adjustment:- tunes the focus and increases the details of the specimen Nose piece :- it is rotating turret that houses the lenses the viewer spins the nose piece to select different lenses Objective lenses:- they are very near to specimen . It ranges power from 40x to 100x Slide :- where the specimen was examined Total magnification ( m) =m1*m2 m1 = linear magnification of objective lens m2 =linear magnification of eye piece lens 3. Haemocytometer is invented by Louis Charles malasezz . It is used for counting blood cells    2 Nutrient agar - streak plate    Casein agar -silk milk palte    Starch agar- starch plate Eosin methylene blue is differential medium positive for only gram - ve 3 At optimum temperature their growth is high where it varies from organism to organism    Disinfectants stops the growth of micro organisms    Antibiotic sensitive organisms are not susceptible in presence of antibiotic producing micro organisms 4. Gram stain - violet blue Acid fast stain- zeil Neelsen carbol fuchsin and alcohol Negative staining- Indian ink or nigrosin Capsule stain -acidic stain :- Congo red and nigrosin -basic stain:- crystal violet,methylene blue , safranin

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