Cloning a gene after isolating it requires which of the following? Select all th

Question

Cloning a gene after isolating it requires which of the following? Select all that apply.

Print il Calculator Periodic Table Question 4 of 5 Genetics: A Conceptual Approach Reading Quiz Pierce 6th Edition MHE/Freeman presented by Sapling Learning Map Cloning a gene after isolating it requires which of the following? Select all that apply. expression of the protein in mammalian cells use of a virus to amplify the cloned gene DNA tel? ligation into a vector identification of the transformants that have the gene of interest transformation of the recipient cells with the vector with the gene of interest

Explanation / Answer

Ligation into a vector.

Transformation of the recepient cells with the vector with the gene of interest.

Identification of the transformants that have the gene of interest.

It is not necessary to express protein mammalian cells. Mammalian cells require virus based vector for gene transfer. Usually bacteria and yeast are used for protein expression.

Viruses are not suitable for gene cloning.

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