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THINKING LAB Identifying Mutations and Predicting Their Consequences Background

ID: 279499 • Letter: T

Question

THINKING LAB Identifying Mutations and Predicting Their Consequences Background As you have seen, a change in a single nucleotide makes the difference between a normal hemoglobin protein and the altered protein associated with sickle cell disease. This table shows nucleotide sequences from different forms of an imaginary gene. In this lab, you will examine these sequences to identify different types of mutations and predict their consequences. Gene type Nucleotide sequence (from template DNA strand) wild (normal) Mutant 1 Mutant 2 Mutant 3 TACCTCTTTCGGGTAACAATAACT TACCTCTTTCGGGTAACTAATAACT TACCTCTTTCGGGTGACAATAACT TACCTCTTTCGGGTAAAAATAACT

Explanation / Answer

Answer=

1]

wild type (normal) = DNA STRAND =

TAC CTC TTT CGG GTAACAATAACT- DNA strand

AUG GAG UUU GCC CAU UGU UAU UGA - mRNA strand

met- Glu - phe - Ala - His -Cys - Tyr - polypeptide

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Mutant 1

DNA template - TACCTCTTTCGGGTAACTAATAACT

mRNA STRAND - AUG GAG AAA GCC CAU UGA UUA UUG A

polypeptide - met - Glu - Lys -- Ala- His

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Mutant 2 ]

DNA strand -TACCTCTTTCGGGTGACAATAACT

mRNA - AUG GAG AAA GCC CAC UGU UAU UGA

Polypeptide - met - Glu - Lys - Ala -His -Cys - Tyr

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Mutant 3]

DNA strand - TACCTCTTTCGGGTAAAAATAACT

mRNA strand - AUG GAG AAA GCC CAU UUU UAU UGA

Polypetide - met -Glu - Lys - Ala - His - Phe - Tyr  

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