Eukaryotic Transcription Questions 1. What kind of RNA does RNA polymerase III t

Question

Eukaryotic Transcription Questions 1. What kind of RNA does RNA polymerase III transcribe? 2. A gene has an upstream sequence at -25 that reads, "TAAAT" This is expressed at a low level. You introduce a nucleotide change at -25 to change the sequence to, "TATAT" What change, if any, would you expect in this gene's transcription level, i.e. is there more or less DNIA made? 3. After eukaryotic transcription is initiated, do TFIID, TFIIB, etc maintain contact with the RNA ogresses down the DNA strand? 4. Does an enhancer tell the RNA Polymerase II where to start transcription? ls 5. What protein is an essential subunit in one of the general transcription factors of all three eukaryotic RNA polymerases?

Explanation / Answer

1. RNA polymerase is the major enzyme, which help to produce RNA from DNA by the process of Transcription. This enzymes have three isozymes RNA polymerase 1, RNA polymerase 2 , RNA polymerase 3.

RNA polymerase 3 transcribe - Transfer RNAs (t RNA), 5S Ribosomal RNA ( 5S r RNA), Y RNA ( one type of small non coding RNA which is component of Ro60 ribonucleoprotein particle), Vault RNAs, 7SK RNA (small nuclear RNA found in metazoans), several micro RNAs, antisense RNAs ( a single stranded RNA which is complimentary to protein coding mRNA) , 7SL RNA ( a part of a signal recognition particle), U6 spliceosomal RNAs ( non coding small nuclear RNA ).

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