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Q 14. When we perform the BAP testing during physiological testing labs, you acc

ID: 280253 • Letter: Q

Question

Q 14. When we perform the BAP testing during physiological testing labs, you accidently forget to incinerate your loop between streaking each new area on the plate. What is the likely result?

While the number of bacteria colonies seen in each area may decrease, the numbers would not decrease enough for individual colonies to form.

Bacterial growth in subsequent areas will be a mixture of contaminants and original bacteria.

There would be no difference in appearance when compared to a plate where the loop had been sterilized between areas.

No bacteria will be able to grow in the subsequent areas of the plate due to contamination of the unsterilized loop.

While the number of bacteria colonies seen in each area may decrease, the numbers would not decrease enough for individual colonies to form.

Bacterial growth in subsequent areas will be a mixture of contaminants and original bacteria.

There would be no difference in appearance when compared to a plate where the loop had been sterilized between areas.

No bacteria will be able to grow in the subsequent areas of the plate due to contamination of the unsterilized loop.

Explanation / Answer

Answer: While the number of bacteria colonies seen in each area may decrease, the numbers would not decrease enough for individual colonies to form.

Incinerating the loop between streaking new areas on the plate removes the bactera from the previous section of streaking. This helps in reducing the bacterial load and forming individual colonies on the plate.