Question 3. Intertidal mussels, Mxtitus.trossuus, collected from the same site i

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Question 3. Intertidal mussels, Mxtitus.trossuus, collected from the same site in Washington, USA, in February and August were exposed for 21h to different temperatures in the lab. Dissected gill tissue was then metabolically labeled at 13°C in the presence of 35S-labelled methione/cysteine before proteins were separated on 10 % SDS-polyacrylamide gels and imaged using standard fluorographic techniques. Individual lanes were loaded with equal counts of radioactivity (3.7 107 Ba), and the locations of molecular mass markers (not shown) are indicated on the right. Visible bands are indicative of protein expression; the darker the band, the more protein expressed. Heat-shock temperature (C) 20 23 25 28 200 kDa 90 kDa 70 kDa 40 kDa February August A. What is/are the independent and dependent variable(s) in this study? B. In response to thermal stress, many organisms increase their production of heat shock proteins, including Hsp70, a 70 kDa molecular chaperone that functions to stabilize other proteins. What do the above results tell us about the relative stress experienced by mussels in the different treatment groups?

Explanation / Answer

A. Independent variable- Temperature,radioactivity

Dependent variables- Protein expressions.They depend upon the changes in the temperature. Because, when thermal stress is present, there is an increase in the shock proteins from the organisms.

B)As we can see, there are darker bands at higher temperature, which means there is more production of shock proteins during high temperature.

Also we can see , as more they wey were wxposed to radioactivity, the bands were becoming more darker, which also indicates the presence of increased proteins.

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