Suppose your research team found a brand new gene that may be involved in the ea

Question

Suppose your research team found a brand new gene that may be involved in the early stages of embryonic development. In studying this gene, your team manages to identify the promoter region and one enhancer region that is 1,000 kilobases upstream of the promoter region. To study this gene’s expression during embryonic development, would you choose to fuse a reporter gene, like GFP, with the promoter region or the enhancer of your gene of interest? Why would you choose that region for your studies?

Explanation / Answer

I would use the promoter region because it is constitutive, meaning that we would have garanteed a continous transcription, so the expression could be more controlled. Also, it could be more specific that an enhancer (the enhancer could act as one for several genes), so the reporter gene could be restricted to certain cells.

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