secondary tertiary quaternary 18. Gel fitration uses resins that allow protein p
ID: 282128 • Letter: S
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secondary tertiary quaternary 18. Gel fitration uses resins that allow protein purfication based on protein's C. D. chargo pH affinity 19. Directional cloning in a plasmid vector usually involves digestion with restriction enzyme(s). three four D, 20. In western blotting, an enzyme-bound is used before addition of the substrate. Primary antibody Secondary antibody C. Biotin D. Bovine serum albumin 21. Automated DNA sequencing uses tagged with different fluorescent molecules. deoxynucleotides dideoxynucleotides Template DNA D. Tag DNA polymerase 22. is the bacteria harboring integrated phage DNA, and integrated phage DNA in that bacteria Prophage/Lysogen is the SPO1/Prophage T7/Lysogen D. Lysogen/Prophage 23. dissociates from filter with ti2 of 30-60 minutes During a filter-binding assay, whereas dissociates at tu2 less than a minute. Holoenzyme/core polymerase Core polymerase/holoenzyme B. TATA boxes are usually present in: A. House-keeping genes 24. Specialized genes Developmentally regulated genes 25. To override transcription attenuation in a trp operon during tryptophan starvation, DNA elements #2 and #3 make hairpin as element#1 is not available due its occupancy by A RNA polymerase C. Ribosome DNA polymerase D. CAP (catabolite activator protein) os all the tRNA molecules i eukaryotic cellsExplanation / Answer
Ans 18 .( A ) size
Gel fractionation chromatography : Dextran gel sephadex is available with various pore size. A mixture is poured over a column of sephadex. The various chemicals pass through the pores and come out of the column with heavier larger molecules do so first followed by progressively smaller sized molecules provided the pores are larger than the size of the largest molecules. This technique is used in determining the molecular weight of proteins by calibrating the column with proteins of different molecular weights.
Ans 19. ( B ) two
Ans. 20 . ( A ) primary antibody
Ans 21.( B ) dideoxynucleotides
Ans 22. ( B) SPO1/ prophase
Ans 23.( A ) Holoenzyme/ core polymerase
Ans 24. ( C ) developmentally regulated gene
Ans 25,( A) RNA polymerase
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