What type of inhibition is this? 1. azapeptides are like peptides but do not hav

Question

What type of inhibition is this?

1. azapeptides are like peptides but do not have amine bonds for serine protease to cleave. They bind to active site and don't allow the formation of tetrahedral intermediates. What type of inhibition is this preforming?

2. Another compound called aspartyl aldehyde can be an inhibitor too. It works by making a reversible tetrahedral intermediate with an active site nucleophilic serine. The effect of this bond is the decrease of available enzyme during the reaction. What type of inhibition is this? Does it break any Michaelis- Mentens rules/ assumptions?

Both of these are binding to Granzyme B, by the way.

Explanation / Answer

1) Azapeptide bind to active site on granzyme B and don't allow for the formation of tetrahedral intermediates. This is a type of competitive inhibition.

2) Aspartyl aldehyde works by making a reversible tetrahedral intermediate with an active site nucleophilic serine. This is an example of non-competetive inhibition.

Both of these follow Michaelus-Mentens assumptions.

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