mustations for Question 6 and 7. The coding strand sequences (in-frame) are show

Question

mustations for Question 6 and 7. The coding strand sequences (in-frame) are shown below. Consider the wild-type S-ATGTTGGGGCGATGGCGCAAA- mutation-1:5-ATGTTGGGGCGATGACGCAAA mutation-2: 5-ATGTTGGCGCGATCCCCAAA 6) What would be the most probalble outcome of the mutation-1? A) silent mutation B) nonsense mutation C) missense mutation D) frame-shift mutation E) addition mutation 7) What would be the most probable outcome of the mutation-27 A) frame-shift mutation B) nonsense mutation C) silent mutation D) addition mutation E) missense mutation 8) Which is NOT true of the Ames test? A) It can be used to screen for cancer causing agents. B) Hidden mutagens can be found by using liver homogenate. C) It uses an auxotrophic strain of Salmonella typhimurium D) It tests for reversions to the wild-type phenotype after exposure to potential mutagens. E) All of the above (A-D) are true. 9) Which pair does NOT physically interact? A) RNA polymerase-Promoter B) Repressor-Operator C) CRP-CAMP D) glucose - cAMP E) Ethidium bromide -DNA 10) An enzyme produced in response to the presence of a substrate is called a(n) A) operator. B) repressible enzyme. C) restriction enzyme. D) inducible enzyme. E) promoter. 11) For the expression of the tryptophan operon, tryptophan itself is a(an) A) inducer. B) repressor C) co-repressor. D) activator E) operator

Explanation / Answer

6) What would be the most probable outcome of the mutation- 1?

B) Nonsense mutation

7) What would be the most probable outcome of the mutation- 2?

A) Frame-shift mutation

8) Which is not true of the Ames test?

E) All of the above (A-D) are true

9) Which pair does not physically interact?

D) Glucose- cAMP

10) An enzyme produced in response to the presence of a substrate is called

D) Inducible enzyme

11) For the expression of the tryptophan, tryptophan itself is a(an)

B) Repressor

12) When do you expect the lac operon is transcribed?

E) Never

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