When activated by the extracellular signal protein platelet-derived growth facto
ID: 29196 • Letter: W
Question
When activated by the extracellular signal protein platelet-derived growth factor (PDGF), the PDGF receptor phosphorylates itself on multiple tyrosines (as indicated in Figure A) by the circled Ps; the numbers next to these Ps indicate the amino acid number of the tyrosine). These phosphorylated tyrosines serve as docking sites for proteins that interact with the activated PDGF-receptor. These proteins are indicated in the figure, and include the proteins A, B, C, and D. One of the cells responses to PDGF is an increase in DNA synthesis, which can be measured by the incorporation of radioactive thymidine into the DNA. To determine which protein or proteins, A, B, C, or D, are responsible for the activation of DNA synthesis, you construct mutant versions of the PDGF receptor that retain one or more tyrosine phosphorylation sites. You express these mutant versions in cells that do not make their own PDGF receptor. In these cells, the various mutant versions of the PDGF receptor are expressed normally, and, in response to PDGF binding, become phosphorylated on whichever tyrosines remain. You measure the level of DNA synthesis in cells that express the various mutant receptors and obtain the data shown in B.
From these data, which, if any, of proteins A, B, C, and D are involved in the stimulation of DNA synthesis by PDGF? Explain your answer.
B. Which, if any, of these proteins inhibit DNA synthesis? Explain your answer.
C. Which, if any, of these proteins seem to have no detectable role in DNA synthesis? Explain your answer.
Please explain
When activated by the extracellular signal protein platelet-derived growth factor (PDGF), the PDGF receptor phosphorylates itself on multiple tyrosines (as indicated in Figure A) by the circled Ps; the numbers next to these Ps indicate the amino acid number of the tyrosine). These phosphorylated tyrosines serve as docking sites for proteins that interact with the activated PDGF-receptor. These proteins are indicated in the figure, and include the proteins A, B, C, and D. One of the cell½s responses to PDGF is an increase in DNA synthesis, which can be measured by the incorporation of radioactive thymidine into the DNA. To determine which protein or proteins, A, B, C, or D, are responsible for the activation of DNA synthesis, you construct mutant versions of the PDGF receptor that retain one or more tyrosine phosphorylation sites. You express these mutant versions in cells that do not make their own PDGF receptor. In these cells, the various mutant versions of the PDGF receptor are expressed normally, and, in response to PDGF binding, become phosphorylated on whichever tyrosines remain. You measure the level of DNA synthesis in cells that express the various mutant receptors and obtain the data shown in B. From these data, which, if any, of proteins A, B, C, and D are involved in the stimulation of DNA synthesis by PDGF? Explain your answer. B. Which, if any, of these proteins inhibit DNA synthesis? Explain your answer. C. Which, if any, of these proteins seem to have no detectable role in DNA synthesis? Explain your answer. Please explainExplanation / Answer
A. By observing the given data, the proteins that are involved in the stimulation of DNA synthesis by PDGF are, A 740, 751, and D 1021
The reason for this conclusion is, presence of these proteins in the mutant showed higher % of DNA synthesis.
B. C 1009 is responsible for the inhibition of DNA, because presence of this protein reduced the % of DNA synthesis enormously.
C. Protein B 771 has no detectable role in DNA synthesis.
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