Which statement is the best explanation of why the researchers added EDTA to the

Question

Which statement is the best explanation of why the researchers added EDTA to the purified alkaline phosphatase solution? 1. EDTA is an agent that binds metal ions and removes them from solution. It was used to remove all possible metal co-factors from the alkaline phosphatase. 2. EDTA is a preservative that helps maintain alkaline phosphatase activity. 3. EDTA is a buffer that provides the correct pH for maximum specific activity of alkaline phosphatase. 4. EDTA is an organic acid that denatured the alkaline phosphatase such that it could no longer interact with its metal co-factor.

Explanation / Answer

1. EDTA is an agent that binds metal ions and removes them from solution. It was used to remove all possible metal co-factors from the alkaline phosphatase.

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