Which proteins do you predict are bound to the promoter in experiment #8? only H
ID: 301323 • Letter: W
Question
Which proteins do you predict are bound to the promoter in experiment #8?
only H and J
only G and H
only G and J
only J
Question 5
Which proteins are likely to act as gene repressors?
both H and J
H
G
J
Question 8
Expression of the Even-skipped (Eve) gene in early Drosophila embryos is under the control of several transcription regulators. In one example, one of the Eve stripes is positioned near the anterior region of the embryo, and its regulatory module contains binding sites for Bicoid and Hunchback (activators) as well as Giant and Krüppel (inhibitors) such that the gene is expressed only in the region where concentrations of the two activators are high and the concentrations of the two inhibitors are low. A reporter gene can be placed under the control of this module, and it can be shown to form a stripe in the same place in the embryo as the corresponding stripe of Eve. Answer the following question(s) based on these findings.
In the Eve regulatory modules, the binding sites for Giant and Krüppel are usually close to, or even overlapping with, those of Bicoid and Hunchback. This implies that Eve expression is regulated by … between the activators and regulators.
Competition
Feedforward regulation
Feedback regulation
Cooperation
Question 10
2,3
2,4
1,4
1,3
Question 8
Some viruses encode a protease that cleaves the translation initiation factor eIF4G, rendering it unable to bind to eIF4E. What is the consequence of this cleavage?
It favors viral protein synthesis by shutting down translation from uORFs.
It shuts down most of the cellular translation machinery, which causes the release of virus particles.
It shuts down IRES-dependent translation, forcing the virus into latency.
It shuts down most host-cell protein synthesis and diverts the translation machinery to IRES-dependent initiation, thus favoring viral protein synthesis.
It favors viral protein synthesis because IRES-dependent translation initiation is inhibited.
Which proteins do you predict are bound to the promoter in experiment #8?
Explanation / Answer
Please find the answers below:
Answer 4: Choice 4 (It can be clearly seen from the tabulated data that binding of protein J to the DNA undoubtedly brings about transcription. However, the transcription is independent whether other protein binds to the upstream regulator or not, hence protein J is the significant in bringing about gene expression)
Answer 5: Choice 3 (the tabulated data shows that binding of protein G undoubtedly prevents gene expression, hence emerged as the repressor protein)
Answer 8: Choice 4 (the information suggests that the regulation between the positive and negative regulatorsis relies upon the expressionpattern of the genes which in turn are overlapping in nature. These genes cannot undergo feedback regulation since expression of one of these genes will modulate the expression of other. Hence, a cooperative pattern of expression takes place)
Answer 10: Choice 3 (the majorgroove of the DNA refers the regions 1 and 4 in the image, hence represents the binding site of the referene molecules)
Answer 8: Choice 3 (It is a very well known fact that the viral genome is translated using host cell machinery. However, in order to undergo translation, it should be favored by host-cell translational machinery. The viruses have evolved a mechanism by which they prevent host-genome translation and favour their own gene translation. This mechanism is performed by IRES-dependent mechanism. Hence, proteolysis of the initiation factor promotes activation of viral genome translation secondary to IRES-dependent mechanism)
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