1) in mammal\'s, the egg cell genome is unmethylated compared to the sperm cell

Question

1) in mammal's, the egg cell genome is unmethylated compared to the sperm cell and both are unmethylated compared to a somatic cell. Which cell has the most number of active genes?

2) list two eukaryotic gene regulation mechanisms that don't involve the promoter region?

3) if you were to inactivate DNA polymerase alpha what effect would it have and in what sort of organism?

4) what characteristics distinguish enhancers from other regulatory elements?

5) why don't eukaryotes use attenuation to regulate their genes?

Explanation / Answer

ANS 1) The utilization of round spermatids that are completely dynamic at the transcriptional level to make zygotes brings up the issue with respect to the under regulation of every single particular quality that are interpreted from the fatherly genome.Therefore, it is demonstrated that protamine 1 and 2 mRNAs, which are particular to the round spermatid organize at the two-pronuclei (6 h) and two-cell (30 h) stages postfertilizatio. The two qualities are completely methylated in round spermatids and sperm however unmethylated in oocytes. At 6 h postfertilization, the protamine 1 and 2 qualities are effectively demethylated, however the demethylation procedure happens more quickly in ROSI than in sperm zygotes. Treatment of zygotes with trichostatin An, a histone deacetylase (HDAC) inhibitor, kept up the protamine 2 mRNAs articulation up to 30 h postfertilization while the DNA methylation status of the quality isn't influenced. In this manner, HDACs are associated with the freedom of protamine 2 mRNAs in ROSI round spermatid injection, two-cell incipient organisms autonomously of the methylation status of the quelled quality.

ANS 2) The center promoter for the most part alludes to the area from the interpretation begin site including the TATA box, which lives around 30 bp upstream of the translation commencement site. This center promoter can't intercede proficient interpretation independent from anyone else. Some vital components close to the promoter, the promoter-proximal components, are found inside 100 to 200 bp of the translation inception site. The CCAAT box works as one of these promoter-proximal cis-acting successions, and a GC-rich section regularly works as another.

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