abcde gy syllabus second Sull llner 201 . Why the necessity oj selecing Gram neg

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abcde

gy syllabus second Sull llner 201 . Why the necessity oj selecing Gram negative enterics? Differentiate and explain the different types of blood hemolysis and their significance. . Under what conditions would 'one' experience blood hemolysis? Week 3: Lab 5. Biochemical Tests Partl Videos: Carbohydrate/Sugar fermentation The Urease Test The Gelatinase Test Starch Hydrolysis The Catalase Test swer the following question a. Why do different bacteria ferment the same carbohydrate differently? b. What is the effect of the fermentation byproducts on the growing bacteria? c. Are fermentation reactions intracellular or extracellular? Explain d. How does proteolysis differ from hydrolysis and digestion? e. What is the essence of the catalase test? 8 6 5

Explanation / Answer

A. Different bacteria have different enzymes for carrying out the process of fermentation. These enzymes work differently, ie, proceed differently and give different products and byproducts.

Hence, different bacteria ferment the same carbohydrate differently.

B. The byproducts cause release of fermentation acids and decrease pH.

This leads to inhibition of growth of many bacteria.

C. Fermentation is an extracellular process. This is one of the first and foremost differences between fermentation and anaerobic respiration, which is an intracellular process.

If Fermentation was an intracellular process, the various harmful acids and substances released would damage or kill the cell itself.

D. Proteolysis is the generally catalyzed (by proteases) breakdown of proteins into smaller peptides and amino acids

Hydrolysis is the uncatalyzed breaking of peptide bond in proteins giving rise to very small monomers or amono acids

Digestion is a physiological process occuring in animals that includes proteolysis and hydrolysis among other processes.

E. The enzyme catalase is produced by the bacteria that respire using oxygen, and protects them from the byproducts of oxygen metabolism.

This catalase test is used to differentiate between aerobic and anaerobic bacteria

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