2. Under which of the following conditions do you expect to see treadmilling of

Question

2. Under which of the following conditions do you expect to see treadmilling of the actin filament? The critical concentrations of F-actin are: (+)-end: 0.12 microM (-)-end: 0.6 microM (1 point)

a. The cytosolic concentration of G-actin-ATP is 0.4 microM, but you have added 0.3 microM of the sequestering protein thymosin to your reaction. Thymosin binds to G-actin ATP in a ratio of 1:1.

b. The cytosolic concentration of G-actin-ATP is 0.15 microM and of G-actin ADP is 0.2 microM.

c. The cytosolic concentration of G-actin-ATP is 0.7 microM, but your reaction also contains profilin, an enzyme that converts G-actin-ADP into G-actin-ATP.

d. The cytosolic concentration of G-actin-ATP is 0.4 microM, but you have also added the (-)-end capping protein tropomodulin to your reaction

e. The cytosolic concentration of G-actin-ATP is 0.1 microM

Explanation / Answer

Treadmilling of the actin filament is senn when e. The cytosolic concentration of G-actin-ATP is 0.1 microM

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