Below is a sequence of double-stranded DNA from a bacterial gene. +1 of the tran

Question

Below is a sequence of double-stranded DNA from a bacterial gene. +1 of the transcribed sequence is indicated and highlighted in bold type. Give the sequence of RNA that would be produced by transcription. Note: you need to refer to your text for the sequence of the prokaryotic promoter. (Assume that when RNA Polymerase runs out of template, it just falls off.) To solve this, you will need the consensus sequence of the prokaryotic promoter

5’- GCGCAAGCTTATCCTGCTGTACCAGACCCTTGGCACCATTATACAGACCTGTACACTTGTCAAATTA-3’

3’- CGCGTTCGAATAGGACGACATGGTCTGGGAACCGTGGTAATATGTCTGGACATGTCAACAGTTTAAT-5’

Explanation / Answer

The sequence of RNA that would be produced by transcription:

5’- GCGCAAGCTTATCCTGCTGTACCAGACCCTTGGCACCATTATACAGACCTGTACACTTGTCAAATTA-3’

RNA-

5’- GCGCUUGCTTUTCCTGCTGTUCCUGUCCCTTGGCUCCUTTUTUCUGUCCTGTUCUCTTGTCUUUTTU-3’

3’- CGCGTTCGAATAGGACGACATGGTCTGGGAACCGTGGTAATATGTCTGGACATGTCAACAGTTTAAT-5’

RNA-

3’- CGCGTTCGUUTUGGUCGUCUTGGTCTGGGUUCCGTGGTUUTUTGTCTGGUCUTGTCUUCUGTTTUUT-5’

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