Assume that the sequence of bases given below is present onone nucleotide chain

Question

Assume that the sequence of bases given below is present onone nucleotide chain of a DNA duplex and that the chain has openedup at the replication fork. Synthesis of an RNA primer occurson this template starting at the base that is underlinedbelow. A) If the RNA primer consists of eight nucleotides, what isthe base sequence? B) In the intact RNA primer, which nucleotide has the free 3'-OH terminus?      3'.....GGCTACCTGGATTCA...5' Any help would be greatly appreciated. Helen Assume that the sequence of bases given below is present onone nucleotide chain of a DNA duplex and that the chain has openedup at the replication fork. Synthesis of an RNA primer occurson this template starting at the base that is underlinedbelow. A) If the RNA primer consists of eight nucleotides, what isthe base sequence? B) In the intact RNA primer, which nucleotide has the free 3'-OH terminus?      3'.....GGCTACCTGGATTCA...5' Any help would be greatly appreciated. Helen

Explanation / Answer

The RNA strand is synthesized in a 5' to 3' direction so the primeris complimentary to these DNA bases 3'...GGTACCT andsynthesis proceeds down in the 5' direction of the DNA strand. A) 5'-CCGAUGGACCUAAGU-3'    The underlinedportion is the RNA primer B)5'-CCGAUGGA-3'OH                 The last 'A' residue of the primer has the free 3'OHterminus.

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