Answer all A. A new analytical extraction method was developed for a DNA analysi

Question

Answer all A. A new analytical extraction method was developed for a DNA analysis in finger print samples. A finger print sample was analyzed six times using the new protocol and found to contain average value of 7580 0 mu g/L with a standard deviation of 10 for DNA A spike recovery was conducted to validate the accuracy of the new method A spike of the blood with 150.0 mu g/L DNA gave a concentration of 7725 mu g/L Assuming the "reference value" of DNA in the samples, is there any significant difference between the result of the finger print analysis and the reference values at 99 9% confident interval? In addition to a sample spike recovery study above, discuss two other analytical protocols to validate the accuracy of the finger print DNA analysis (Note, this is a discussion question, therefore, detailed explanation is required before you can obtained full credit)

Explanation / Answer

To calculate significant difference at 99.9% confident level, we use following formula:

If confident level = 99.9% then significant p value = 0.1% or 0.001

Any p value of test below 0.001 makes significant observations, in this test.

[Reference value - Test value/ Reference value] x 100

[7725 – 7580 / 7725] x100 = 0.0188 x 100 =.188 %

Alternatively, p value = 0.0188 which is higher than 0.001; so at 99.9% confidence level, the test provide no significant difference.

We can testify the fingerprinting DNA analysis by using following two methods:

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