10 Circular dichroism (CD) is a technique used for determining the secondary str

Question

10 Circular dichroism (CD) is a technique used for determining the secondary structure (a-helix and B-pleated sheet of a protein. The authors took CD spectra of the protein at varying temperatures at various wavelengths. Then they plotted the results obtained at 220 nm. The results are an indication of the integrity of the secondary structure of the BN protein. A decrease from 100% indicates a loss of secondary structure integrity. The results are shown below. a What do these data tell you about the stability of the protein at various temperatures?

Explanation / Answer

The image shows a loss in percentage of secondary phase with temperature. There is a sharp change at near 10 0 C. and then the percentage is almost same and the again it goes down around 80 0 C to a lower percentage of 60 %. This can be related to loss of all secondary structures or a particular secondary structure present in the protein.

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