Which of these BEST describes the way a polyacrylamide gel should be placed in a

Question

Which of these BEST describes the way a polyacrylamide gel should be placed in a MiniProtean running box (like the one you used in lab for the SDS-PAGE competency)? The wells should be at the top with the shorter glass plate towards the user. The wells should be at the top with the shorter glass plate away from the user. The wells should be at the top with the shorter glass plate towards the outside of the running box. The wells should be away from the user and the bottom of the gel should be toward the user. The shorter glass plate should touch the green rubber gasket when the gel is clamped into position.

Explanation / Answer

Answer: Option B is correct.

Explanation:

In SDS-PAGE, the gel plates must be placed in such a way that the buffer is in direct contact with the gel so that electricity will move the sample. The sample also must run through the gel i.e. the wells must be towards to negative electrode.

This is achieved by placing wells at the top so that we can load samples into it. The shorter plate is placed away from the user and towards the buffer side so that it is in direct contact with the buffer.

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