Note: Respond To All Questions From Part A To C se GenBank record to Respond For

Question

Note: Respond To All Questions From Part A To C

se GenBank record to Respond For All Questions From Part A To C Below type-"genomic DNa" /db xref-"taxon:562" 286. .291 /regulatory class "promoter" /note-"-35 region" 310..316 /regulatory_ clas"promoter" /note="-10 region" 322..324 /note-"put. transcription atart region" 330..333 A. What are the simple citations (use format above) for any publications about this gene? How long is the entire gene? what are the sequences of the start and stop codons? regulatory B. C. regulatory x01714 1609 b DNA LoCUS linear BCT 23-OCT-2008 DEFINITION E. coli dut gene for dUTPase (EC 3.6.1.23) (deoxyuridine migc feature regulatory S-triphosphate nucleotidohydrolase) /regulatory clas ribosome_binding site" ACCESSION x01714 VERSION REYWORDS SOURCE X01714.1 dUTPa; unidentified reading frame Escherichia coli /note-"put. rrNA binding site" 343.798 /note="unnamed protein product; dUTP- CDS ase (aa 1-151)" ORGANISM Escherichia coli Enterobacterales; REFERENCE 1 (bases 1 to 1609) Bacteria; Proteobacteria; Garmaproteobacteria; Enterobacteriaceae; Escherichia Lundberg. L.G., Nucleotide sequence of the structural gene for Eacherichia coli K-12 /codon start-1 /transl tablc-11 /protein-id="CAA25859. 1" /db xref-"GOA: P06968" /db xref-"InterPro:IPRO08180" /db xref=" InterPro:IPR008181" /db xref-"PDB: 1DUD" /db xref-"DB: 1DUE" AUTHORS Thoreason,H.O., Karlstrom, O.H and Nyman, P.O TITLE duTPase of /db_xref-"PDB:1U JOURNAL EMBO J. 2 (6),967-971 (1983) PUBMED 6139280 Data kindly reviewed (25-NOv-1985) by L Lundberg FEATURES /db_xref=" PDB: 1SYL /db xref="PDB :2HR6" Location/Qualifier 1. .1609 /organism-"Escherichia coli" /db xref-"UniProtKB/swiaa-Prot: PO6968

Explanation / Answer

A. The best way to cite a paper is to go to google scholar and type the title of the paper. In this case, it will be "Nucleotide sequence of the structural gene for dUTPase of Escherichia coli K-12". The title is given on the page for the GenBank entry. The google scholar will give the results. Go to the appropriate result and click the symbol for cite ". It will give all formats of citation. One of the simple citations are in MLA style so here is the citation:

"Lundberg, Lennart G., et al. "Nucleotide sequence of the structural gene for dUTPase of Escherichia coli K?12." The EMBO journal 2.6 (1983): 967-971."

B. The length of the gene is given in the first line itself where it says 1609bp. So, the length of the gene is 1609 bp.

C. The transcription start site is given in the misc_feature from 322..324. So, in the gene we will go to that site and find out the transcription start region. Here the sequence from 322..324 is acg. So, the transcription start site is acg as opposed to site 343 atg site which is translation start site. As the codons represent the translation start site, therefore, the start codon is atg present at 343..345. To check this you can also look for the first CDS. The first CDS starts from 343. So, the start codon is at the position 343..345 i.e. atg. The last coding sequence in the GenBank entry is given from 905..1540. The last 3 codons in this sequence is taa. So, the stop codon is taa present from 1538..1540. To quickly navigate the GenBank entries remember, that each block is 10 bp and 60 bp constitute a line. To summarise, the start codon is atg at position 343..345 and the stop codon is taa at 1538..1540.

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