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Note: Respond To All Questions From Part A To C mol_type-\"genomic DNA\" /dlb xr

ID: 3164737 • Letter: N

Question

Note: Respond To All Questions From Part A To C

mol_type-"genomic DNA" /dlb xref-"taxon: 562" 286..291 /regulatory_class-"promoter" note-"-35 region" 310..316 regulatory_clas"promoter" note-"-10 region" 322..324 note-"put. transcription start region" 330. .333 se GenBank record to Respond For All Questions From Part A To C Below A. What"regulatory elements" have been identified and where are they regulatory located? B. What is the sequence of the -35 element of the promoter? C. How long is(are) the protein-coding region(s) of the gene? (This crazy regulatory gene appears to have two coding regions!) x01714 1609 bp DNA LOCUS linear BCT 23-OCT-2008 DEFINITION E. coli dut gene f?r dUT Pa e (EC 3.6.1.23) (deoxyuridine misc feature regulatory 5'-triphoaphate nucleotidohydrolase) x01714.1 Eacherichia coli Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriaceae; Eacherichia. /regulatory class-"ribosome_binding site" ACCESSION X01714 VERSION KEYWORDS dUTPase; unidentified reading frame SOURCE note-"put. rRNA binding aite" 343..798 CDS /note="unnamed protein product; dUTP- ase (aa 1-151)" ORGANISM Escherichia coli Enterobacterales; REFERENCE 1 (ba5e51 t?1609) and Nyman, P.o dUTPase of codon start 1 /transl_table=11 /protein-id-"CAA258 59. 1 " db xref-"GOA: P06968" db xref-"InterPro:IPR008180" /db xref="InterPro : IPR008181" /db xref-"PDB:1DUD" /db xref=" PDB : 1DUP" AUTHORS Lundberg, L.G., Thoresson, H.O., Rarlstrom, o.H TITLE Nucleotide sequence of the atructural gene for Escherichia coli K-12 db_xref "PDB:1EUW" /db xref-"PDB: 1RN8" JOURNAL EMBO J. 2 (6), 967-971 (1983) PUBMED 6139280 COMMENT Data kindly reviewed (25-NOV-1985) by L Lundberg Location/Qualifiers 1..1609 /organism= "Escherichia /db xref=" PDB :1SYL" /db xref=" PDB :2HR6" /db xref=" PDB :2HRM db xref-"Uni?rotKB/wiaa-Prot: P06968 coli"

Explanation / Answer

The accesion number is for the gene - E. coli dut gene for dUTPase (EC 3.6.1.23) (deoxyuridine 5'-triphosphate nucleotidohydrolase)

The regulatory elements in the gene include-

2) The sequence of -35 promoter is ' ttgagc'

3) The gene has 2 CDS regions but the dUTPase protein coding region lies in region from 343..798 nucleotides.

The other CDS doesnot code for dUTPase protein

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