Bio3 Sample Lab Quiz #2 Write your frst name only in the blank in the upper righ

Question

Bio3 Sample Lab Quiz #2 Write your frst name only in the blank in the upper right of both sides of this page. (7 Fill in the blank (1 pt each blank) is a virus that is commonly grown in an egg. are methods used to grow mammalian viruses in the lab Short Answers formation is a type of CPE (cytopathic effect) associated with certain types of viruses What are syncytia? (2 pts) 1b. What types of viruses are likely to be able to create this CPE? (2 pts) 2. During the phage typing experiment, we spotted water in addition to phage on our plates. What was this called (be specific) and why did we do this? (2 pts) 3. During the phage typing experiment, we used TSA plates to grow our E coli. However, the composition of this agar was relatively unimportant. Why? (2 pts) Lab Math 1. What are the following dilutions? (1 pt each) 1a. 100 pL of turpentine into 99.9 mL of paint 1b. 1 uL of polymerase into 99 UL of buffer

Explanation / Answer

Ans 1. Influenza

Isolation of influenza virus using using egg has been done even before the culture techniques became popular. Embryonated chicken eggs still remain the most commonly used method for growing influenza virus.

Ans 2. Tissue culture, Inoculation in embryonated eggs, Inoculation in lab animals

?Ans 1a. Syncytia are cytoplasmic masses of multinucleated cells formed by fusion of cells.

Ans 1b. Morbillivirus (measles virus), Paramyxovirus (parainfluenza virus), Ribulavirus (mumps virus) and Pneumovirus (respiratory syncytial virus)

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