A) Here, we will simulate an experiment using a standard curve to estimate the c

Question

A) Here, we will simulate an experiment using a standard curve to estimate the concentration of a newly discovered molecule, Hollinase, in an unknown sample. The concentration of molecules can be given as a mass per volume, when you don't know the formula weight. In the chart below, calculate the amount of standard Hollinase at a known concentration (0.5 ug/ul) and water to combine to make l ml of solution at each concentration listed. If you need a reminder about how to do this, see the appendix "Biological Math".

Explanation / Answer

Let’s calculate for 1ng/uL

Stock = 0.5ug/uL = 500 ng/uL

Required = 1ng/uL

required Volume = 1 mL =1000uL

C1V1= C2V2

(stock)   (required)

V1=C2V2/C1

V1 = (1ng/uL X 1000 uL)/ 500 ng/uL

= 2 uL

2 uL of stock + 998 uL of diluent

Sol con ng/uL

vol of Hollinase uL

Vol of H2O uL

Total vol mL

0

0

1000

1

1

2

998

1

2.5

5

995

1

5

10

990

1

10

20

980

1

==

Sol con ng/uL

vol of Hollinase uL

Vol of H2O uL

Total vol mL

0

0

1000

1

1

2

998

1

2.5

5

995

1

5

10

990

1

10

20

980

1

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