You look more closely at the sequence of the gene from Question #2, and you find

Question

You look more closely at the sequence of the gene from Question #2, and you find evidence that it is a kinase (an enzyme that phosphorylates other proteins). You therefore want to try to produce large amounts of this protein so that you can do in vitro biochemical experiments with it. 3. What specialized kind ofvector do you need to put the DNA coding for this protein into in order to make the protein in cells? What would be a good host to try if your goal is to make large quantities of your protein? What are some possible problems that might occur using this host? a. b. c.

Explanation / Answer

A.)

Commonly used Expression vectors is pET vectors for expressing the gene of interest.

B.)

E.coli can be used as host system for the production of large quantities of recombinant protein. It is because it has a fast growth kinetics. It has a doubling time of 20 minutes.

C.)

No or low expression, inclusion body formation, protein inactivity are some of the problems that might occur while using this host.

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