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point mutations in the hemoglobin gene (called hemoglobin S, C, and E) on suscep

ID: 3176464 • Letter: P

Question

point mutations in the hemoglobin gene (called hemoglobin S, C, and E) on susceptibility of humans to malaria, as compared to people with the non-mutant hemoglobin gene (in essence a “control”; labeled "W" for wild-type). To do so, they took blood samples from 40 people (10 wild type, 10 S-type, 10 C- type, and 10 E-type) and measured the number of red blood cells infected by the malaria protozoan per microliter. Do any of the hemoglobin mutations confer better resistance to malaria as compared to the wild-type, non-mutant control? Please use spss if you have it Mutation Group Infected Cells 22 27 20 125

Explanation / Answer

I have ANOVA study by taking use of all three type of hemoglobin gene (called hemoglobin S, C, and E) on susceptibility of humans to malaria, as compared to people with the non-mutant hemoglobin gene.

First by doing ANOVA, single factor study between S group and Wild type (W) group. Here are the result:

We can see here that P value = 0.001128, i have taken significance level 0.05 and we can see that it is under that significance level and also F value(14.96) is greater than F(critical)(4.41) so it can be said that this doesn't provide better resistance to malaria in comparison to wild-type, non-mutant control.

Similarly, next i have done ANOVA study between C group and Wild type (W) group. Here are the result:

We can see here that P value = 0.000922, i have taken significance level 0.05 and we can see that it is under that significance level and also F value(15.67) is greater than F(critical)(4.41) so it can be said that this doesn't provide better resistance to malaria in comparison to  wild-type, non-mutant control.

Similarly, next i have done ANOVA study between E group and Wild type (W) group. Here are the result:

We can see here that P value = 0.000922, i have taken significance level 0.05 and we can see that it is not under that significance level and also F value(4.19) is greater than F(critical)(4.41) so it can be said that this type of mutation (E) provide better resistance to malaria in comparison to other mutation type(S and C) with respect to wild type non-mutant control.

Anova: Single Factor SUMMARY Groups Count Sum Average Variance S 10 105 10.5 18.5 W 10 38 3.8 11.51111 ANOVA Source of Variation SS df MS F P-value F crit Between Groups 224.45 1 224.45 14.95779 0.001128 4.413873 Within Groups 270.1 18 15.00556 Total 494.55 19