What is the smallest distance two points can be separated and still resolved usi

Question

What is the smallest distance two points can be separated and still resolved using light microscopy? 20 nm 0.2 mu m 2 mu m 200 mu m TRUE OR FALSE: Mammalian cells can be grown on a Petri disks. True False The pink color added to the DMEM media is an indicator of nutrients availability optimal temperature pH of the media Humidity level in the incubator Choose the best combination in the following statement: optimal conditions for growing mammalian cells are incubators with 37 degree C, 5% CO_2, 10% O_2 37 degree C, 5% CO_2, humidity 30 degree C, 10% CO_2, humidity Room temperature, 5% Air, pH 7.4 We purchase immortalized mammalian cells for biomedical education and research Wall Mart Hospitals CCTA ATCC

Explanation / Answer

12. Wavelength. resolving power of a microscope depends on wavelength of light used to illuminate the specimen, Angular aperture and refractive index.

13. 0.2 um

14. True. mammalian cells can be grown in Petri dishes with the specific cell culture media containing glucose and other essential factors for the vitality of cells

15. Pink color added to the DMEM is the indicator the pH of the medium.

16. 370C temperature, 5% CO2 and humidity is the best condition for the cell to grow in the cuture media.

17. ATCC (American type culture collection) is a leading world cell repository.

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