Did you obtain isolated colonies? If not, explain why? If so, how can you determ

Question

Did you obtain isolated colonies? If not, explain why? If so, how can you determine if the colony is a pure colony? In the streak plate technique, how are the microbes dilluted and spread dut to form individual colonies? On what spread plate(s) did you obtain isolation? Once you obtained isolation at a particular spread dillution, did you continue to have isolation on subsequent dillution plates? Is this what you expect? Explain why or why not? What is the purpose of the ethanol is the spread plate technique? What was the purpose of previous laboratories nutrient media preparation, aseptic techniques, streak plate and spread plate?

Explanation / Answer

1) Ethanol is antimicrobial which is used to sterilize the spreader used for spread plate technique. The spreader should be aseptic before it is used to take the inoculum because only the desired type of strain is allowed to grow.

2) In streak plate technique, a loop is used to take the inoculum and streaked in the plate to give some patterns. At the end of streaking, individual cells from the loop will be removed to form individual colonies.

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