Did you think your DNA would be visible after you had broken open you cells? Why

Question

Did you think your DNA would be visible after you had broken open you cells? Why or why not? Where do you think you would find proteases in your body? In which cellular compartment do you expect to find your genomic DN A? Match the outcomes on the left with the laboratory steps on the right. Harvest the cells Dissolve cell membranes precipitate the DNA Break down proteins Gently chew the insides of your mouth and then rinse vigorously with water Add protease, incubate a. 50 degree C Mix in a detergent solution Laver cold alcohol over cell extract

Explanation / Answer

4) DNA is contained in the chromatin fibres within the cell nucleus. These strands of chromatin are very thin, and are not visible in uncondensed form. they are like swirling strands within the nucleoplasm. During interphase, the chromatin fibres condense to form chromosomes and they are visible only then.

5) dietary proteins are digested in the stomach and intestine. the stomach mucosa has chief cells or peptic cells which secrete protein digesting enzyme pepsin breaking proteins into proteoses and peptones.

further down the GI tract these are broken down into peptides and amino acids by trypsin in the small intestine which is secreted by Crypts of Lieberkuhn.

6) genomic DNA is found in the cell nucleus.

A- harvests the cells

B- break down proteins.

c- dissolve cell membranes.

D- precipitates the DNA

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