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Clearly state the null and alternative hypothesis and your assumptions. The perc

ID: 3233187 • Letter: C

Question

Clearly state the null and alternative hypothesis and your assumptions.

The percent reduction in blood sugar a certain time after injection of insulin into rabbits is given below. A group of 24 rabbits was divided at random into six groups of four rabbits each, and each rabbit received an injection of insulin. Two factors were involved, the dose at three levels and the preparation of insulin in two ways, A and B. Analyze this using a two-way analysis of variance for a factorial design (repeated measurements). (10 points).

                                                            Dose Level

Preparation I ii                     iii

            A                      17                 64                 62

                                    21                 49                 72

                                    49                 34                 61

                                    54                 63                 91

            B                     33                 41                 56

                                    37                 64                 62

                                    40                 34                 57

                                    16                 64                 72

Explanation / Answer

Two way anova with m observations per cell:

H01 : There is no significant difference in blood sugar after injecting insulin due to doses.

H02 : There is no significant difference in blood sugar after injecting insulin due to preparation.

H03: There is no significant difference in blood sugar after injecting insulin due to interaction affect between doses and preparation.

Alternate hypotheses:

H11 : There is a significant difference in blood sugar after injecting insulin due to doses.

H12 : There is a significant difference in blood sugar after injecting insulin due to preparation.

H13: There is a significant difference in blood sugar after injecting insulin due to interaction affect between doses and preparation.

1

2

3

A

17

64

62

21

49

72

49

34

61

54

63

91

B

34

41

56

37

64

62

40

34

57

16

64

72

1

2

3

A

141

210

286

B

127

203

247

Analysis:

Grand Total = 1214

CF = G2/N = 12142/24 =61408.17

Total sum of squares = Raw S.S – CF = 172 + 642 + … + 342+412 +…+642+722 - 61408.17

= 8113.833

M = 4; p=3 ; q=2

1

2

3

T.j.

T.j.2

A

141

210

286

637

405769

B

127

203

247

577

332929

Ti..

268

413

533

1214

738698

Ti..2

71824

170569

284089

526482

Sum of squares due to treatments (dose) = Ti..2/mq – CF

= 526482/4*2 - 61408.17 = 4402.083

Sum of squares due to variety (preparation) = T.j.2/mp – CF

=738698/4*3 - 61408.17 = 150

Sum of squares due to interaction = ( Tij.2/m – CF)- SST-SSV

Tij.2 = (141)2+(210)2+(286)2+(127)2+(203)2+(247)2 = 264124

S.S due to interaction = (264124/4)- 61408.17-4402.083-150 = 70.75

SS due to error = 8113.833-4402.083-150-70.75 = 3491

Anova table

Source of variation

Degrees of freedom

Sum of squares

M.S.S

Variance ratio

F at 5% level

Dose

p-1 =2

4402.083

=4402.083/2

=2201.0415

F1 = 2201.0415/193.944

=11.3489

F(2,18) =3.5546

Preparation

q-1=1

150

=150/1

=150

F2=150/193.944

=0.7734

F(1,18)=4.4139

Interaction

(p-1)(q-1)=2

70.75

=70.75/2

=35.375

F3 = 35.375/193.944

=0.1824

F(2,18)=3.5546

Error

18

3491

=3491/18

=193.944

Total

mpq-1=23

Inference:

1)Since Fcal =11.3489 > Ftab = 3.5546, we reject the null hypothesis and conclude that there is significant difference in blood sugar after injecting insulin due to doses.

2) Since Fcal =0.7734< Ftab = 4.4139, we accept the null hypothesis and conclude that there is no significant difference in blood sugar after injecting insulin due to preparations.

3) Since Fcal =0.1824 < Ftab = 3.5546, we accept the null hypothesis and conclude that there is no significant difference in blood sugar after injecting insulin due to interaction effect between dose and preparation.

1

2

3

A

17

64

62

21

49

72

49

34

61

54

63

91

B

34

41

56

37

64

62

40

34

57

16

64

72

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