lIn rabbits, expression of certain genes (such as the Himalayan allele of the ty
ID: 3268910 • Letter: L
Question
lIn rabbits, expression of certain genes (such as the Himalayan allele of the tyrosinase gene) is sensitive to changes in environmental temperature. Suppose you set up 4 different climates in the lab: (25°, 27°, 29°, 31° C) and put 5 Himalayan rabbits in each environment. After 3 hours of exposure, you sample the rabbits, assay for tyrosinase activity, and record level of enzyme activity for each animal. What test should you do if you want to determine whether tyrosinase activity is significantly different at different temperatures? and Why? ( How can i simpify this to help me deternine which test i should use)
Explanation / Answer
Answer
One-way ANOVA (Analysis of Variance) with equal number of observations (5) per cell will be the appropriate test.
The 4 different temperatures are 4 treatments.
Y = Level of enzyme activity for each of 5 rabbits form the response variable.
The model is: Y = µ + i + , where µ = common effect, i = effect of ith treatment (temperature) and is the error term which is assumed to be Normally distributed with mean 0 and variance 2.
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