When the ? 2 -AR is activated, a signaling cascade is initiated that leads to cA

Question

When the ?2-AR is activated, a signaling cascade is initiated that leads to cAMP dependent phosphorylation of protein X. Cells were transfected with control silencing RNA ( siRNA) or ?-arrestin siRNA to prevent expression of ?-arrestin. The cells were then incubated in epinephrine and, at various times, aliquots of the preparation were prepared for Western blotting using an antibody directed against phosphorylated protein X.

What is a likely explanation for the differing pattern of protein X phosphorylation in the ?-arrestin

Explanation / Answer

Cells that were transfected with beta-arrestin siRNA to prevent expression of beta-arrestin shows the production of phosphorylated protein X; whereas cells in control siRNA shows depletion of phosphorylated protein X synthesis after 10 mins of incubation with epinephrine. This clearly shows that beta-arrestin has significant effect on the synthesis of protein X.

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