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Response optimization is used to determine the optimal conditions for recovery o

ID: 3383598 • Letter: R

Question

Response optimization is used to determine the optimal conditions for recovery of a sample for HPLC. The three factors are: A- Solvent (Hexane or Ethyl Acetate); B- pH (8 or 10); and C- time (30 or 60 minutes). The two response variables are Recovery and Variance. What are the optimal conditions for this process?

Run

Recovery

Variance

(1)

65.3

4.5

a

81.3

4.8

b

53.3

3.7

ab

69.9

5.5

c

61.8

3.7

ac

77.45

6.9

bc

73.9

3.6

abc

89.9

6.2

Run

Recovery

Variance

(1)

65.3

4.5

a

81.3

4.8

b

53.3

3.7

ab

69.9

5.5

c

61.8

3.7

ac

77.45

6.9

bc

73.9

3.6

abc

89.9

6.2

Explanation / Answer

What are the optimal conditions for this process?

recovery of 65.3 and variance 4.5

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