E. coli strain AX has the genotype Amp S bio+ trp+ leu- . E. coli strain AY has
ID: 34259 • Letter: E
Question
E. coli strain AX has the genotype AmpSbio+ trp+ leu-.
E. coli strain AY has the genotype AmpRbio- trp- leu+.
Part A
On which type(s) of media will the AX strain grow?
Select all that apply.
In an experiment similar to the Lederberg and Tatum experiment, researchers grew two strains of E. coli in complete medium.E. coli strain AX has the genotype AmpSbio+ trp+ leu-.
E. coli strain AY has the genotype AmpRbio- trp- leu+.
The researchers then plated the bacteria on different media types.Part A
On which type(s) of media will the AX strain grow?
Select all that apply.
a) leu- b) trp- c) bio- d) leu-trp-bio- + ampicillin e) complete medium f) complete medium + ampicillinExplanation / Answer
The genotype of E. coli strain AX is AmpS bio+ trp+ leu-, which indicates that the strain is susceptible to ampicillin and require leucine from the medium. This is because the gene required to synthesize the leucine is absent in the AX strain E. coli. Thus, it cannot grow in the presence of ampicillin.
Thus, the correct options among the following are b, c, and e.
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