ELISA Simulation Sample Color Test result positive control negative control Pati

Question

ELISA Simulation Sample Color Test result positive control negative control Patient A Patient B Patient C Patient D Patient E Patient F Dark purple Light green purple Light green Light green Light green purple Light green Positive Negative Positive Negative negative negative positive negative 1. What basic principles of antibody-mediated immunity are utilized in an ELISA assay? ELISA assays are based on the principles that antibodies are produced in response to infection and that these antibodies are designed to specifically target particular antigens and bind tightly to them 2. How does an ELISA indirectly detect infection by a disease-causing agent? 3.What is the function of the secondary antibody and chromogen in an ELISA?

Explanation / Answer

1; Alreasy given the solution

2; indirect ELISA is used to detect the infection of the patient by detecting the presence or absence of antibodies against the particular pathogen. While during an entry of pathogen, the specified antigen of the pathogen is recognised by the specialised cells of immune system and they directly produce the corresponding antibody, which circulates the blood stream for a definite period of time to eliminate the pathogen, these chemicals can be directly detected by the indirect ELISA test.

3; Detection of antigen and antibody complex can be carried out with secondary antibody and chromogen.

4; Due to variance results, we should perform atleast three test for the best accurate result, also we can ensure the reproducibility of the test results,

5; The rate of color formation is directly depends upon the amount of specific antibody in patients blood.

The followings are the reason for the reduced antibody level in serum

6;

7 Answer will vary

Related Questions

Navigate

• Browse (All)
• Browse E
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.