2. Assume there is an object on the left side of the field that you want to brin

Question

2. Assume there is an object on the left side of the field that you want to bring to the center (that is, toward the apparent). In what direction would you move your slide?

3. The area of the slide seen when looking through the microscope is the ?

4. Why should the light be dimmed when looking at living (nearly transparent) cells ?

5. If, after focusing in low power, you need to use only the fine adjustment to focus the specimen at the high powers, the microscope is said to be ?

6. You are using a 10x ocular and a 15x objective, and the field diameter is 1.5 mm. The approximate field size with a 30x objective is _______ mm..

7. If the diameter of the low-power field is 1.5 mm, an object that occupies approximately a third of that field has an estimated diamter of ________ mm.

Explanation / Answer

Ans 2) To the left. The slide will be moved towards the left so that there is good focusing and the object can be visualized better.

Ans 3) Field – It is basically the area or the diameter of the field present under the optical light microscope. The area which is directly under the microscopic lens.

Ans 4) Increases contrast – The light when it is dimmed while observing the living cells helps in increasing the contrast which enhances the visibility.

Ans 5) Parfocal – These are generally those lens which stay in focus despite a change in the focal length or magnification.

Ans 6) 0.75 mm

Ans 7) 0.4 mm since the whole of the diameter is 1.2mm and the answer can be found out by binding of 1/3rd of 1.2mm

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