3) Cylinder made up of a semipermeable membrane contains a 60mM CaCl2 solution a

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3) Cylinder made up of a semipermeable membrane contains a 60mM CaCl2 solution and is surrounded by a saline of 100mM NaCl. Calculate the osmolarity of the two solutions and determine the direction in which water will flow. Will the volume in the to be an increase or decrease? Lab Section Number Name Prelab Exercises 1. Blood glucose concentration is typically around 90mg/dL. What is its molar concentration? 2. The average pH of blood, extracellular fluid and cytosol is around 7.4. What is the H concen- show work below) tration of these fluids? 3. A cylinder made up of a semipermeable membrane contains a 60mM CaCl2 solution and is surrounded by a saline of 100mM NaCI. Calculate the osmolarity of the two solutions and determine the direction in which water will flow. Will the volume in the tubing increase o decrease? Ca)180 nesmols/L wntur wl Flo the volume ntubi 4. How many orders of magnitude difference exists for Ca* concentration across a plasma membrane? (1 order of magnitude = 10 fold, 2 orders of magnitude = 100 fold, etc.) Lab Exercises

Explanation / Answer

3).

The osmolarity of a solution is = osmotic coefficient of solute* number of particles into which the solute can dissociate* the molarity of solute

The osmolarity of 60mM CaCl2 is = 3*60 = 180 mOsm/L

The osmolarity of 100mM NaCl is = 2*100 = 200 mOsm/L

It is given that the calcium chloride is inside the cylinder and sodium chloride is outside, both are separated by the semipermeable membrane. Water flows from the lower solute concentration (indicated by lower osmolarity) to the higher solute concentration.

Thus, the water moves towards outside of the cylinder, so the volume in the cylinder decreases

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