During the experiment executed on March 7th members of the laboratory group went
ID: 3513477 • Letter: D
Question
During the experiment executed on March 7th members of the laboratory group went outside to collect a sample of twelve leaves. This leaves are next to the building on the immeditate left of the entrance facing Southside Dining Hall. When the leaves were collected six of them were from the plant that was in a shadow and the other six were receiving natural sunlight. Both samples come from the same type of plant. After collecting the leaves, we went back to the laboratory to examine the leaves and see if the plants that were in the sun had more stomata open than the ones in the shadow. For that, we use tape, clear nail polish (brand is not relevant), microscope, the leaves we took, clean slides, scissors and plastic slide holder.
The process to check how many stomata a leafs have is very simple. The first step is collecting the leaves, follow that you need to make sure to obtain all the information relevant to that collection meaning time, date and location of the sample. Once you get to your laboratory, split both samples to make sure that you do not mix the ones that were in the sun with the ones in the shadows. Now you will get the nail polish and paint the leaf underside (make sure is a thick layer), then you will get the tape and cut a piece of it with the scissors then place it in the leaf. Make sure to don’t put your fingerprints or anything else their otherwise the experiment will not work. Now that you have your leaf in the tape let it sit for a few minutes. Once the drying is done, you will take the leaf out very carefully and place the tape on the clean slide. Now you will set the slide with the leaf under the microscope and use the different lenses of the microscope until you get to 400x. Once you can see all the stomata in the leaf start counting how many you see after that, you will use the following formula (Unit of Stomata at 400x= #of stomata/ 0.12mm2) this will give an approximate amount of stomata that you have in the leaf. Now repeat this steps with each one of your leaves and record your results.
Summarize the data from the experiments without discussing their implications.
Include tables and figures
Table legends should be on top of the table.
Figure legend should be below the figure
Make sure the legend is descriptive
Talk about the general trends and differences and not on trivial details
Leaf Lab File Edit View Insert Format Data Tools n 100% . $ % .0.00 123. fx Light Leaf Number of Stom. Calculated Stoma 5 Leaf 46 36 46 57 64 71 383 6 Leaf2 Leat 3 8 Leaf 4 Leaf5 t0 Leaf& 383 475 533 502 12 Shadow Leat Number of Stomi Calculated Stoma 47 45 53 60 392 375 442 500 46Explanation / Answer
The table given above have six leaf tapes each for leaves exposed to light and leaves kept in shadow.
The leaves exposed to light the 400X value increase with the increase in stomata.
The leaves kept in shadow the 400X value differs according to the number of stomata. This is because how many stomata have been exposed to the light when they are kept is the shadow is difficult to know. So when the put under the 400X microscope their count differs.
In both cases the the 400X value is directly proportional to the number of stomata.
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