Please help clearly explain/answer animal physiology question. it is a 2 part qu

Question

Please help clearly explain/answer animal physiology question. it is a 2 part question (one cannot be answered with out the other).thank you

Question:

(Q3)For ANS 100, we have an opportunity to go to the Arctic (thank-you anonymous donor!) and we come across a bunch of frogs that are all the same species. You are interested to know whether these frogs can use some of the same cellular mechanisms as the Woodland, CA and Costa Rican frogs to cope with differences in environmental temperature. How might you go about doing this? Hint: You have access to temperature incubators that can be adjusted to any temperature you want

(Q4)After your run your experiment in Question 3, you then isolate lactate dehydrogenase (LDH) enzyme from the three groups of frogs to look at the substrate binding affinity (measured by calculating the Km). Will the Km of these frogs at those given temperatures be the same (or similar) or very different? Please explain your answer.

Explanation / Answer

The metabolism in an animal body consists of various cellular mechanisms which is further guided by different enzymes . Enzymes work at a certain optimum temperature and is inactivated at both very low and high temperatures . Thus , ceasing the cellular mechanisms needed for proper body functioning .

Frogs are cold blooded animals . They cannot maintain their inner body temperature according to the outside temperature .

Therefore they either go into hibernation during winters and summers .

3) Here as we have access to temperature incubators , we will adjust the temperatures according to that in Woodland , CA and Costa Rica one at a time . Then we will incubate the frogs in the incubators at the respective temperatures . We will allow some time for the frogs to adapt to the incubator temperature and study the different behaviours of the frogs to know whether the cellular mechanism and hence metabolism is whether the same , increased or decreased at that temperature .

4) Km is a constant for a given temperature . Km is the measure of how easily the enzyme can be saturated by the substrate . At lower or higher temperatures , the enzyme gets denatured and inactivated . So at different temperatures , the activity of the enzyme will be different and hence Km also . So, Km will be different at different temperatures foe those frogs.

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