HM 1360 Spring 2018 ab 5 Osmosis and Diffusion Pre-lab worksheet Name 1. A flask
ID: 3518247 • Letter: H
Question
HM 1360 Spring 2018 ab 5 Osmosis and Diffusion Pre-lab worksheet Name 1. A flask contains two compartments (A and B) with equal volumes of solution separated by a semipermeable membrane. A is initially an aqueous solution of 10% (w/v) glucose and B is initially an aqueous solution of 20% (w/v) glucose (a) (0.5 point) label A and B in terms of relative tonicity (b) (0.5 point) After two hours, the volume of solution in one compartment is visibly larger than the volume of the solution in the other compartment. Draw a diagram of the flask and label which side initially contained solution A and which side initially contained solution B (c) (0.5 point) Explain which component(s) of the solution has moved in which direction overall? (d) (1 point) Based on the fact that glucose and water are both polar, what do you think is the key permeability factor for this semipermeable membrane? 2. (1.5 point) A flask contains two compartments (A and B) with equal volumes of solution separated by a semipermeable membrane, same membrane as Q1. A is initially an aqueous solution of 10% (w/v) methanol and B is initially an aqueous solution of 20% (w/v) methanol. After long waiting, the final levels of the liquids did not change. Explain what has happened by osmosis or simple diffusion. What would be the final concentration of A and B?Explanation / Answer
Answer 1
a):-
A is relatively hypotonic to B. B is relatively hypertonic to A. [Relative tonicity is the tonicity of solutions separated by semi-permeable membrane, in relation to each other].
b) and c):-
The liquid from Side A (10%w/v glucose) moves through semipermeable membrane to the Side B (20%w/v glucose). The water movement occurs till both solutions acquire same tonicity. Thus, Side B would have higher volume but same tonicity as Side A.
d):-
The passage through semipermeable membrane is not based on polarity of the substance, but it is based on the molecular size. Some hydrocarbons and oxygen can pass through the semipermeable membrane. Glucose is larger molecule than water and hence glucose cannot pass the semipermeable membrane even if it is polar.
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