Chromatographic assay reveals that the total amount of T3 in the blood is 10 ?g.

Question

Chromatographic assay reveals that the total amount of T3 in the blood is 10 ?g. You find that the various bound forms of the hormone are: T3(free) = 0.5% T3 ~ albumin = 12 % T3~TBG = 69 % and T3~pro-albumin = 18.5 % Target tissue: heart whose Bmax of T3 receptors = 76 fmol of T3 receptor/mg protein (1 fmol = 10-15 mol), where you have 100 ?g protein (total protein of which T3 receptors makes only a tiny fraction) in your heart assay. MW = 68 kDal [Dasmalopatra et al., Receptor, 2(4):213-223, 1992]. Note: You won

Explanation / Answer

76fmoles = 5168 pico grams = 5.168 nano grams

Free T3 available in blood = 50 nano grams

T3 that would bind to 100 micrograms protein = 0.5168 nano grams

Amount of T3 available = 50 - 0.516 = 49.484 nano grams

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