We can schematically represent DNA synthesis as illustrated below. The two illus

Question

We can schematically represent DNA synthesis as illustrated below. The two illustrations are intended to represent the same molecule at two different times. The polarity of each strand ?s indicated with an arrowhead at the 3' end. The short jagged lines are meant to indicate the RNA primers that initiate synthesis. Geneticists study everything by means of mutations, and DNA replication is no exception. The roles of many of the proteins involved in replication were first identified using temperature-sensitive mutations. Proteins with temperature-sensitive mutations will work nearly normally at some temperature (the permissive temp) yet not work at all at a higher temperature (the restrictive temp). For each of the proteins below, predict the outcome (sketch a diagram like the second one above) if E. coil is grown at the permissive temperature until time=t and then switched to the restrictive temperature for delta t. Explain your prediction. Even though DNA synthesis will eventually come to a halt if any of these enzymes are non-functional, you may assume that all other processes that don't directly require the function of the damaged enzyme will continue for at least the length of the experiment. (12 pts) DNA polymerase III DNA ligase Primase

Explanation / Answer

DNA polymerase 111 holoenzyme is the enzyme, which is responsible for replication in E. coli. It consist of primer, which is initiated form 5

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